My sexual identification is based on size. Last night I discovered the thin one dead. It was expected, so I am not torn up. Besides the factors you mentioned, I have also noticed that light can determine the intesity of color in frogs. After lights out I have notice that a lot of my frogs do darken in color, this is especially true of my treefrogs.

Thanks, for the insight on their natural enviroment. The only info I have to go on as far as natural history are filed guides. So thanks.