American Toad Questions!

[JButera]

Try picking him up with your thumb and index finger just behind his front legs, a male toad will let out a very audible release call. I have tiny little oak toads and all the males squeak when I pick them up in that manner. I've heard that infertile females have a 'I'm not ready' call; however, its very faint compared to the males' call.

[Yelikosium]

She doesn't make any noise when I do that! I was under the impression that you wouldn't be able to tell that way until a toad has reached sexual maturity, but if what you say is true, that's awesome! Thanks for the info.

[JButera]

Yeah, I'm not too sure about the maturity thing either, but I do have male toads that seem a little too young to breed yet they squeak all the time when picked up. I'm not sure if sexual maturity needs to be reached since it's a non-breeding call. It's basically an "I'm a male so get off me" call.
https://www.youtube.com/watch?v=ifq7d18EgGE