I'd look into the possibility of illness. Not getting out of the water is a classic sign of being sick.
I noticed you just switched to reverse osmosis? That can be hard on a frog's kidneys and it lacks elements they need. See here= http://www.caudata.org/cc/articles/Spring_water.shtml
If the water had been cloudy because of some kind of bacteria bloom or heavy metal that might have been a problem too.
When did you switch to reverse osmosis? Because if the frog started showing symptoms a little bit after, it was probably that. If it stopped eating before or during the switch it might be something else.





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