Quote Originally Posted by LilyPad View Post
I have bioactive substrate in my vivariums and still would not condone allowing accidental ingestion of substrate. I still see bits and pieces of stuff that I would not want in my frogs' intestines. It's from stuff that helps to encourage drainage when planting a naturalistic vivarium.
In the interests of trying to help, it seems that you haven't got your bio-active substrate set up correctly for your tree frogs. Let me take you through it:

Drainage is accomplished by use of a drainage layer, not bits and pieces mixed in. A layer of clay balls, egg crate or something similar should be at the bottom covered by a mesh. On top of this mesh is your soil, usually a mixture of coco fibre and fine topsoil in which your springtails etc live. The frogs should at no point come into contact with the drainage layer so there shouldn't be any 'bits and pieces of stuff' that they can accidentally ingest that would cause impaction issues. I strongly suggest you remove what you have in there and start again following these guidelines. I see now why you are trying to rely on a glass bowl for feeding, but it isn't necessary if the vivarium is set up correctly. By and large tree frogs prefer to hunt in the trees of course, but they will take prey from the ground too so it's good to ensure that substrate is fit for purpose.
Healthy frogs have a good mechanism for ensuring they do not ingest harmful foreign bodies (otherwise they would have a hard time of it in the wild with no glass bowls!), but we can and should in the captive environment ensure that foreign bodies are not in the substrate to begin with thus negating the (albeit small) chance of impaction problems.