Red-eyed tree frog is dark green and in the water dish two mornings in a row

[LilyPad]

Agree with Eli, get that humidity UP. Do you have a full screen top? If so, you may need to cover 1/2-3/4's of it. Covering 3 sides of the tank should help them relax a little. Turn off the red light at night, a lot of reds are sensitive and will not wake up to eat. It's normal for them to go darker at night but they should be a nice bright green during the day.

[threetreefrogs]

Answers to many of the questions you asked are stated in my post...species, size of terrarium, # of inhabitants, temp, humidity level, dietary concerns, etc.

I've up'ed the humidity to 75%, as recommended. The three red-eyed tree frogs were received through the mail from Backwater Reptiles 9 days ago. They were introduced to their new 18x18x24 terranium with all new materials (tank and all accessories had been rinsed with warm water). Temp always hovers around 75 degrees by way of a double dome light that shines with white UVB light by day, red light by night (to maintain temp). The tank is going to be cleaned for the first time this weekend. I mist several times a day with distilled water to maintain the humidity (which is difficult to do at this dry time in Nebraska). There is a water dish for soaking which is filled with tap water that is treated with Zoo Med Reptisafe Instant Terrarium Water Conditioner and changed daily. I have the felt-like subtrate (I can't remember what it's called, but it's designed for this purspose) covered with moss to help maintain moisture. The terrarium is adorned with a nice-sized climbing log and an artificial plant. The back is covered with a scenic sticker and the two sides are covered with black paper to reduce stress. Frogs are offered 1/4" crickets daily, which have been dusted in calcium and vitamin D3. They have yet to eat. I place them in a plastic bowl and keep track of count. All three frogs are adults and were captive bred. They handled very infrequently...only about one time each to show them the dish with crickets so they'd know where to go to hunt. Hands were washed prior. Currently, the frogs are kept in my classroom. I manage to keep the noise level down (keeping from stressing the frogs has been a great incentive) but I still wonder if it's too "busy" for them. My husband is helping me haul them home this weekend where the daytime activity is at a minimum.

I contacted Dr. Frye today, as the female frog had new symptoms this morning. She was found in the water dish again and now has bright green spots...consistent with a bacterial infection, from what I've read. The following meds are on their way:
Metronidizole
Silversulfadiazene
Baytril
Panacur

In addition, I'm to be watching for a fecal sample to test for parasites. Might I add, Dr. Frye is amazing. I emailed him with my concerns and some pics this morning and he got back with me in less than 5 minutes!! The other two frogs appear healthy. Their lack of eating is a concern for me, but from what I've read they can go for up to 2 weeks. Any additional advice on quarantining, etc, is welcomed. I'm worried about our little Charlee. (The other two are Michael Jackson, as named by my son, and Lola). I'd love to include a photo, but this site is not allowing me to do so.