over and over again. water bowls and water features are a HUGE problem!!!! Constant Carbon use in the water is HORRIBLE for frogs!!!! carbon strips the water of everything! in aquariums, fish can lose their protective slime coat and develope pits in their skin if carbon is constantly used!!!! frogs are even more sensative. Carbon does NOT remove bacteria from the water!!!! it is a porous substate that can develope and help colonize bacteria! if there is a bacteria problem in the water ususally you wont see it, in fact the water may even look "very clean" to the eye! You must have the whole thing Bleached!!! bleach is the only sure fire way to kill alll bacteria. OR if you have a water feature then you MUST install an Ultraviolet sterilizing unit (Aquaultraviolet and lifeguard units are the most relaible brands on the market today) to the system. but this requires the water feature to have an external filter unit, like a canaster filter. the U.V. unit can be made inline with the return line from the canaster unit that goes back to the enclosure. Sorry for your lose! If you have a water bowl or water feature you MUST keep it sterile. frogs need the same heat and humidity that bacteria cultures explode in growth under.