Thanks for all the replies, I guess that settles it, I was already leaning towards the separate tanks, but wanted to know for sure.

Some of you have said the Mossy frogs had travelled far but these are all captive bred frogs, and yes these will be breeding colonies. I will be trying my hand at breeding Pacman, Milk Frogs, Mossy frogs, and Red Eyed tree frogs. This is an expansion to our reptile breeding.