the handling of frogs....
the safest advice is don't touch, do not disturb.
that being said, frogs(like other critters) that are around/handled by humans since early on and semi-regularly are not very stressed by it. My Big girl Stitch and older boys Eric and Tyler were in petco for months before I got them, hardly handled, hid in back, only ever had to tolerate kids tapping the glass, they will skip eating for one night after handling or tank cleaning. my others that I've had since they were penny sized are used to me and dont even open their eyes/wake-up if I peel the leaf out from under them onto my hand. I also find that giving them a good misting around the night of rain or snow helps kick their mindset to breeding instead of dwelling on the weird looking tree janitor that cleaned their room.