That was the scariest part for me. I was always afraid that I wouldn't have enought because I wouldn't culture on the right schedule. I'll tell you what I do. That may help!

Ok.. I started off making a new culture weekly. At the time I had 5 froglets. I ended up with more than enough flies that way.. and I was doing a daily feeding since they were young. I tapered off a bit and starting making cultures now as soon as my last one booms.

For example.. lets say I made a culture today. I would add a few flies (10-20 is what I choose to go with) into the new culture. Once flies have sprouted from the new culture then I make another new one from that. It usually takes 5-10 days for the boom.. but it insures that you have new flies going into your new culture. I've noticed that the 'boom' time can vary with different media used.

So what I would do is start off making a new culture each week on the same day of the week each time. That way you get into a nice schedule. After a few weeks you will have the hang of it and be able to decide if you can go as far as 10 or so days between them.

I'm currently culturing hydei, mels, and turkish gliders for my frogs.. so my specific schedule wouldn't work for you. That is why I suggested trying to make a weekly one and then work out the kinks .

From the time the culture booms you may be able to feed from it for a few weeks.. up to a couple of months in most cases. I always had 4 going at once and would get rid of my oldest one each week to replace with a new culture.

Be sure to not allow the lids to touch on your cultures. That is an easy way to transfer mites to what may be a mite free culture. Also.. all vivs have mites of some type in them (frogs find them to be tasty snacks) but keeping your cultures around your tanks isn't a good idea. I choose to keep mine on a shelf in the laundry room to make sure that doesn't happen.

Editing to say that I reread my post and one part may be confusing.. When I said I had 4 going at once.. I didn't mean that I started 4 at the same time. You have 2 cultures now. If you made a new one that would put you up to 3. Another one a week from then would make 4.. and when you make another the following week, that is when I would get rid of my oldest culture. Let me know if that doesn't make sense and I'll try to explain a bit better .