Im not an expert so im just going to ask for you to send some pictures of the leg so heatheranne, flybyferns, or some of the other experts can see what the problem is. And one other question, what kind of neosporin did you use? The kind with pain killers in it will kill the frog (it will say PAIN RELIEF next to the neosporin logo) but if you used just the normal original neosporin than thats not the problem.