Baby ACFs with twitchy legs

[Gemma]

I apologise for the length of this...

A few nights ago I noticed one of my baby ACFs involuntarily twitching his/her legs (I have since found this old thread which has a video of someone else's frog doing the same thing: http://www.frogforum.net/aquatic-cla...aking-leg.html - like the second frog it shows, not the first). I didn't notice the other frogs in the tank showing any signs of this, but then again I wasn't looking at the rest very carefully since I thought it was an illness affecting just the one frog.

The next night, I noticed that about half of the frogs in the tank were doing the same thing. They had no other visible signs of illness, and they were using their legs normally and swimming around as lively as they usually do despite the twitching. I'm not sure how long the twitching lasted but I noticed it at about midnight and they were still doing it when I woke up in the night (3am ish).

The thread in the link above suggests that it could be due to a nutritional deficiency. The only thing that I've done differently with them lately is to do with their diet.

I had been feeding them Zoo Med Aquatic Frog Food and the occasional treat of frozen bloodworms since they morphed (I gave up on Reptomin a while ago because it was just too messy to feed that amount of froglets). With Zoo Med as their staple food, the frogs were growing quickly and they never had any problems with the food. Recently I ran out of Zoo Med so I fed the froglets frozen bloodworms for about 5 days while I waited for the new jar of Zoo Med that I ordered online to arrive in the post.

The day the new jar of Zoo Med arrived was the day I noticed the first frog twitching. I had fed them the food from the new jar just a few hours before. The next day when I noticed them twitching, that was also a few hours after I'd fed them the new Zoo Med food. The day after that I fed them bloodworms again because I was paranoid about the Zoo Med food, and they haven't twitched since. I haven't given them any more Zoo Med.

I bought the old jar of Zoo Med, which was fine, from Amazon. When it arrived the seal on it was intact.
I bought this new jar from a business seller on eBay. When it arrived, the seal underneath the lid had already been peeled off and was just resting underneath the lid. I'm wondering if the pellets could have somehow become contaminated or gone off because the airtight seal had been removed. The label doesn't say anything about using them within so long after opening though, and the date on the lid says 2015.

It might not have been that, but I haven't done anything else differently and the frogs haven't done it again since I stopped feeding them that food.

Do you think this could have been the cause, or does anyone have any other theories that I might not have thought of (other than issues PH, temperature, NH3, NO2, or NO3)? The only other thing I can think of is I have very soft water, but the twitching has now stopped even though the water is obviously still soft.

[Gemma]

This is completely unrelated to this topic but I thought I'd share it.

Over the last couple of days I've caught some of the froglets amplexing a few times! They're only 3 months old (from being eggs). They definitely take after their father