Metro is a good medication. I have used it for most of my rescues. My vet always had me give it via topical drops also.
At this point you can just wipe the hospital tank down with hot dechlorinated water. Of course, be careful not to burn your little fella. I would soak Twiggy in his pedialyte dilution soak (in a mini critter keeper with a air-vented lid) while I cleaned out his cage. I had pre-made boiled dechlorinated water and put it into an empty spring water jug and allowed it to cool a bit. I'd use it to wipe out the enclosure daily with paper towels. I used the same pre-made water from the jug to mist his tank and new paper towels, and to fill his water bowl with (also changed daily). I used the water to moisten his paper towel for picking him up to feed him too. The goal was to minimize bringing in any new bacteria into his hospital tank. I would put Twigs back in when the tank was cool. I'd also wash my hands and put on powder-free gloves before doing any tank care, and again wash them when done. Diluted 10% bleach is fine to use, but you have to rinse very thoroughly and then rinse again with dechlorinated water.
Tree frogs change color. Spots and/pr patchy discolorations are generally an infection of some type. They will persist and likely worsen until properly treated.
The normal changing of colors can briefly make them look mottled or patchy, which could look like infection. How you will know, is the color change will finish making them look "normal" again, along with proper eating, pooping, soaking and they will have normal activity levels, energy, mobility, sleep/wake cycles and no further skin discolorations or lesions. Essentially they will look and act normal after the skin color change.
Even the way the light reflects on them or areas of shading from light can alter their colors. They will always return to a overall same shade of green when the lighting or darkness is even against them.
Here are some examples of the ways the light hits them differently and the way they can alter their skin as camouflage. It's quite amazing really.
Is this photo you can see Serena's solid green hue (minus her "freckles")...
In this photo you can see the bluish color where the lighting is brightest from that side of the tank...
Here you can see he has 3 different shades of green...
This photo is a good example of a color change in progress, incomplete. Here it looks as of he has an infection, though he does not and he finished the color change within a few minutes.
We have to monitor our frogs behavior and eating/defecating habits along with assessing their skin to determine when something is wrong. Skin alterations that persist throughout the day are cause for immediate alarm and need to be treated quickly. A good time to observe their skin is when they're asleep and late at night when they are hunting. It gives you 2 color variations to compare. Now, keep in mind I have a retf. Most tree frogs can exhibit the same color changing responses. Skin alterations tend to show up most when the frog is dark. Another thing to be aware of is when green frogs tend to linger in a dark brown discoloration when they are not against a brown background, it could be a sign that they are stressed and/or sick. Still though, behaviors will help to determine such.





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