When cultures start out there are usually between 50 to 100 flies added to the cup. The cup will slowly begin to be covered in little pods which eventually larva will emerge from. The pods will continue to fill the sides of the cup as the larva begin to turn into flies. Your cups will become almost black inside with flies. They will continue to produce until the media has depleted.
Have a funnel on hand, it comes in handy when wanting to calcium or vitamin dust the flies.
I clean and reuse the cups a few times. Oh and get some mite paper to place the cups on to reduce the chance of mites.