every animal in your tank will most likely dia. Springs, isos, frogs, etc. but springs are easy to replace if you have a culture going.

** always keep a big culture of springs on hand. I started with a culture from Josh's frogs that i moved to a 6qt container that has grown to 1000's and 1000's. I just add a few hundred once a week


http://www.dendroboard.com/forum/care-sheets/57367-building-using-co2-generator-2.html#post497332