Good to know, although I have been using Joshs media for about a year so I forgot what that step was I just remembered that there was an extra one. When I started I was not sure if the yeast made a difference or not so I just did it.

I do like your advice about doing the back up culture. I have had a couple cultures crash over the last year but I have the luxury (although I should not rely on it) of having 3 shops within 5 miles of my house that carry fruitflys.