The bacteria are usually found in well established or "cycled" fish tanks. They reside mostly between the gravel. I am just guessing, but believe you would still have to use some type of similar underlying gravel or substrate which would have to be in water. I don't know if placing the substrate over top would work or not. I've never tried it.
If I were to try it I'd use rocks or gravel that is larger than the amphibians you wish to house. And I would recommend a bubbler or a waterfall for circulation. My gravel is small because I house red eye tree frogs who do not eat in or near their water so there is little concern for ingesting the gravel. You would still have to do water changes bc the toxins would still build up. My current terrarium functions on that basis, but I still do water changes biweekly for my pond area and daily for my frog's water bowl.
Stagnant water will allow unwanted other bacteria, algae and nitrates (waste byproducts) that the healthy bacteria cannot keep up with. Still the good bacteria are helpful.
But, you don't have to buy such bacteria. You could start a tank with rocks, dechlorinated water, a bubbler and a simple fish. It will take about 4 to 6 weeks to establish or "cycle". You may notice the water will start to become cloudy and a little stinky. This is the blooming of the bacteria. After that time, you can take out the fish and do a full water change, but only gently rinse the rocks as to not kill the good bacteria. Some recommend only a 75% water change then. Your good bacteria will be ready.
I would recommend monitoring the ph of the water and clarity for a bit longer before placing your frogs in the tank.
Look up cycling an aquarium on the web. I'm sure you'll find some good articles. I learned from my sister and other buddy, and a little trial and error.
My tank has been well established for years and now only needs partial water changes. I also have a waterfalls filter. FYI, submerged filters will often use a charcoal filter and also will bloom with the healthy bacteria.
Here is a picture of my tank used in a variety of ways over the years using the same principle and also a filter.
I hope this helps! Good luck! Keep us posted on what you decide![]()