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It became necessary to update my large viv I had thought the substrate had sunk so far into the water it had gone sour however when I stripped the bottom out of the tank all was fine and the only source of the smell that I could find was the plug in the manual drain. To explain I have two drains on the tank one attached to the side where I turn a tap and let gravity and suction do the work and another larger tube which stands upright in the tank and with a baster I can suck out any remaining water ...