Sexing American toad metamorphs

[PaisleyT]

Hi! This summer I had several metamorphic American toads and I was wondering if it is possible to know the sex of them. I know for adults it is possible to determine if they are males or females according to the colour of their throats, presence of nuptial pads, and/or if they make calls. When is the earliest that these metrics can be used? Do they have to be reproductively mature? If so, are there any other tricks for determining the sex of young toads (or even tadpoles)?
I have pictures of all of them. Any help would be greatly appreciated!
Thanks in advance
- Paisley