When can you tell a pyxie frog's gender?

[Teh Frog Whisperer]

How old/big does a pyxie frog have to be for you to be able to accurately tell the gender?

[John]

According to scientific research (see the African Bullfrog article for a reference), the two sexes begin to differentiate at 6.6 cm (that's just over 2.5 inches).

[Teh Frog Whisperer]

And is there any way to be absolutely sure about the gender when the frog reaches that size? Because I want to be 100% sure that my frog will be a male.

[John]

No, no guarantees I'm afraid. I'm confident that I could sex a 4 inch / 10 cm pyxie in person, probably through photos too.