D. hydei Culturing Issues

[Lynx]

A few weeks ago I managed to successfully culture hydei from a handful of flies left in a couple of pet store vials. From that I made 2 more cultures one day apart. The older of the 2 had lots of dead flies withing a couple days and no larvae, while the newer did great. I even used some of those flies to start a third culture. One is beginning to hatch new flies, and the newest is starting to pupate. So, myquestions are:

1. How long after flies begin to hatch should I reculture? It's my understanding that the females hatch first and take less time to reach sexual maturity. Anyone know how long after the initial hatching the males emerge?

2. How long after they hatch should I wait to feed? I tried to wait a few days with the original before making another culture so that I'd have males and females, but after 3 days, it was getting really crowded in there, so I did end up feeding some to the frogs. I don't want to end up feeding all the females and still ending up with a skewed sex ratio.

Sorry if it's a silly question, but I haven't been able to find an answer, and the froggies really seem to prefer the hydei to the melanogaster at this point. In fact, they don't even want to bother with the smaller flies anymore.

[bshmerlie]

Wow...that's an issue I never really paid that much attention to. I guess I make mine a week after the culture blooms. Once the culture takes off I wait a couple of days until it looks real good then start to feed from it. Then a few days later I make my new cultures. At that time the culture is going really well. Hydei does not reproduce as fast as the Melanogaster so if I were you I would always keep a culture or two of the Melanogaster around as a backup. Of course depending on how many frogs you are feeding. Just be careful to ensure a culture crash doesn't cripple you.

[John]

1: I start new cultures every 7 days. Hydei cultures don't produce for long. As long as there are significant numbers of new flies in a newly producing culture you shouldn't need to fret about sexes after about 48 hours.

2: I feed pretty soon after production starts, just don't feed more than 75% of he flies each day so the sexes will balance out.

[Lynx]

Thanks much, guys.

if I were you I would always keep a culture or two of the Melanogaster around as a backup.

I always have 2 or more melanogaster cultures going, and will definitely continue to do so just in case.

I start new cultures every 7 days

I do as well, at least with the melanogaster. I only had the one hydei culture to work with initially, but now with 3 going, one a week from the first 2, I think I can do the same there.

Oh! does anyone mix flies from new and older cultures when making a new one?

[John]

There's no reason why you can't mix them, but be aware that mites often travel on the bodies of fruit flies, so the older cultures are more likely to have more mites in them, so using flies from the old cultures could increase the number of mites in the new cultures.

[Lynx]

Thanks for the heads up. Despite my best efforts, I have a serious mite problem. I have to discard cultures after about the weeks, sometimes less. I don't know if they can be cultured out or if I need to start from scratch.

[berksmike]

A good method is the first dust the flies with your calcium powder and then sieve the powder away with a vey fine mesh sieve. This will hopefully dislodge the mites. Also using mite papaer helps - as well as washing your hands after handling cultures.

On the plus side most dart frogs will readily take mites as a food source - my azureus love them so I keep a few mite infested cultures in the shed for that purpose (well away from my active fly cultures tho)